InSolution Y-27632; Sterile-Filtered; Cell Culture-Tested

Application

InSolution™ Y-27632, Sterile-Filtered, Cell Culture-Tested, CAS 146986-50-7, is a 10 mM sterile-filtered solution of Y-27632 in H₂O. Acts as an inhibitor of Rho kinases (Ki = 140 nM for p160ROCK).

Biochem/physiol Actions

Primary TargetROCK

General description

Ready to use, cell culture-tested, suitable for stem cell applications. Acts as a highly potent, cell-permeable, reversible, and selective inhibitor of Rho-associated protein kinases (K_i = 140 nM for p160ROCK). Also shown to improve survival of human stem cells especially during cell culture passage and manipulation.

A 10 mM (2 mg/591 µL) sterile-filtered solution of Y-27632 in H₂O.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Li X. et al, et. Al., 2009. Human Reproduction, 24, 580.Chitaley, K., et al. 2001. Nat. Med.7, 119.Davies, S.P., et al. 2000. Biochem. J.351, 95.Narumiya, S., et al. 2000. Methods Enzymol.325, 273.Hirose, M., et al. 1999. J. Cell Biol.141, 1625.Maekawa, M., et al. 1999. Science.285, 895.Uehata, M., et al. 1997. Nature.389, 990.

Packaging

Packaged under inert gas

2 mg in Glass bottle

Physical form

A 10 mM (2 mg/591 µL) sterile-filtered solution of Y-27632 (Cat. No. 688000) in H₂O.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.

Warning

Toxicity: Standard Handling (A)